中华皮肤科杂志 ›› 1999, Vol. 32 ›› Issue (3): 150-151.

• 论著 • 上一篇    下一篇

连接酶链反应用于淋病的诊断

徐克沂1, Carolyn M Black2   

  1. 1. 北京地坛医院性传播疾病防治中心, 100011;
    2. 美国疾病控制中心性传播疾病部
  • 收稿日期:1998-07-21 修回日期:1998-09-14 出版日期:1999-06-15 发布日期:1999-06-15

Diagnosis of Neisseria Gonorrhoeae with Ligase Chain Reaction

XU Keyi1, Carolyn M Black2   

  1. Beijing Ditan Hospital, Beijing 100011
  • Received:1998-07-21 Revised:1998-09-14 Online:1999-06-15 Published:1999-06-15

PDF

309

赞

摘要： 目的 评价连接酶链反应(LCR)技术对淋病诊断的意义。方法 对在计划生育门诊就诊的330例女性患者进行细菌培养及LCR检测,对两项结果不符的标本进行聚合酶链反应检测,实验结果应用EpiInfo软件进行分析。结果 330例患者中30例LCR检测阳性(91%),27例培养阳性(82%),13例两项结果不符者进行聚合酶链反应检测。结果表明LCR的敏感性及特异性分别为89%及99.7%.结论 LCR用于淋病的诊断具有较高的敏感性及特异性。

关键词: 淋病, 培养, 聚合酶链反应, 连接酶链反应

Abstract: Objective To evaluate the value of ligase chain reaction (LCR) in the diagnosis of gonorrhoea.Methods A comparative evaluation of LCR and cell culture was performed in 330 women in a family planning clinic. For resolving discordant specimens between the two tests, polymerase chain reaction was used. The result of tests was analysed with EPIINFO software.Results 30 of 330(9.0%) women had positive LCR results and 27 of 330(8.2%) were positive for cell culture. 13 specimens were discordant for LCR and cell culture. The sensitivity and specificity of LCR in the diagnosis of gonorrhoea were 89% and 99.7% respectively.Conclusion This study shows that LCR has a higher sensitivity and specificity for the diagnosis of gonorrhoea.

Key words: Neisseria Gonorrhoeae, Culture, Ligase chain reaction, Polymerase chain reaction