中华皮肤科杂志 ›› 1999, Vol. 32 ›› Issue (1): 9-11.

• 论著 • 上一篇    下一篇

系统性红斑狼疮患者外周血淋巴细胞bcl-2的表达及临床意义

刘亚光1, 尹培达1, 石成钢1, 赵湘茹2, 孔庆瑜1, 黄必军3   

  1. 1. 中山医科大学附属第一医院风湿科 广州 510080;
    2. 中山医科大学附属第一医院教学实验中心 广州 510080;
    3. 中山医科大学附属第一医院肿瘤研究所 广州 510080
  • 收稿日期:1997-10-16 修回日期:1998-05-11 出版日期:1999-02-15 发布日期:1999-02-15
  • 基金资助:
    国家自然科学基金资助!(39770706)

Expression of bcl-2 in Lymphocytes from Patients with Systemic Lupus Erythematosus and Its Clinical Significance

LIU Yaguang1, YIN Peida1, SHI Chenggang1   

  1. Division of Rheumatology, Kidney Institute, First Affiliated Hospital, Sun Yat Sen University of Medical Sciences, Guangzhou 510080
  • Received:1997-10-16 Revised:1998-05-11 Online:1999-02-15 Published:1999-02-15

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摘要： 目的 为了探讨bcl-2基因在系统性红斑狼疮(SLE)发病机制中的作用及临床意义.方法 应用逆转录-聚合酶链反应(RTPCR)检测31例SLE患者外周血单一核细胞(PBMC)bcl-2 m RNA表达水平和流式细胞仪双标记法分析其T、B细胞bcl-2蛋白表达.结果 活动期SLE患者PBMCbcl-2 m RNA表达水平明显升高,占556%,且活动期SLE患者CD3+、CD4+和CD8+T细胞亚群bcl-2蛋白表达明显高于非活动期SLE患者、正常人对照组和其他疾病组,而CD19+B细胞bcl-2蛋白表达在各组之间并无统计学差异.结论 T细胞bcl-2异常表达在SLE疾病活动的过程中起着重要作用,检测SLE患者外周血T淋巴细胞bcl-2的表达水平可作为SLE的活动性指标.

关键词: 红斑狼疮,系统性, 细胞死亡, Bcl-2

Abstract: Objective To investigate the role of bcl-2 expression in the pathogenesis of systemic lupus erythematosus (SLE) and its clinical significance. Methods The bcl-2 mRNA levels in peripheral blood mononuclear cells (PBMC) from 31 SLE patients were tested by reverse transcription polymerase chain reaction (RT PCR),and the bcl-2 protein levels in T and B cells were also tested by two colour cytofluorography. Results bcl-2 mRNA levels in PBMC increased significantly in 10 cases of active SLE,accounting for 55.6% of active SLE patients. Compared with those in inactive SLE patients,normal controls and other non SLE patients,the bcl-2 protein expression in T cell subsets (including CD3+,CD4+ and CD8+) increased significantly in active SLE patients. However,there was no statistical difference in bcl-2 protein levels in CD19+ B cells among all groups. Conclusion The abnormal expression of apoptosis related bcl-2 in T cells might play an important role in disease activity of SLE,and the determination of bcl-2 expression levels of peripheral T lymphocytes might be used as an index for disease activity of SLE.

Key words: Lupus erythematosus,systemic, Apoptosis, Bcl-2