中华皮肤科杂志 ›› 1997, Vol. 30 ›› Issue (5): 324-326.

• 论著 • 上一篇    下一篇

系统性红斑狼疮患者外周血单一核细胞CD23的表达

李希清1, 凌绍贤1, 王斌2   

  1. 1. 中山医科大学孙逸仙纪念医院皮肤科;
    2. 中山医科大学免疫教研室
  • 收稿日期:1996-11-07 修回日期:1997-02-02 出版日期:1997-10-15 发布日期:1997-10-15

Study of Expression of CD23 on Peripheral Blood Mononuclear Cells in Patients with Systemic Lupus Erythematosus

Li Xiqing1, Ling Shaoxian1, Wang Bin2   

  1. Department of Dermatology, Sun Yat Sen Memorial Hospital, Sun Yat Sen University of Medical Sciences, Guangzhou 510120
  • Received:1996-11-07 Revised:1997-02-02 Online:1997-10-15 Published:1997-10-15

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摘要： 为了揭示B细胞中CD23的表达与SLE发生发展的关系及在SLE发病机理中可能的作用,我们应用ABC免疫组化法和斑点核酸杂交技术对SLE患者外周血单一核细胞(PBMC)CD23蛋白和mRNA表达进行了检测.结果显示:30例SLE患者PBMCCD23蛋白表达显著增高(P<0.01),且与疾病活动呈正相关关系(rs=0.3814,P<0.05);具有不同ANA、抗dsDNA抗体水平,有无伴肾损、脑损的SLE患者,PBMCCD23表达均无显著性差异(P均>0.05);单纯使用皮质类固醇激素治疗或和其它免疫抑制剂联合治疗的SLE患者,PBMCCD23表达亦无显著性差异(P>0.05).20例SLE患者PBMCCD23mRNA表达较正常人显著增高(P<0.01).经治疗病情稳定后,CD23蛋白和mRNA表达均降至正常(P均>0.05).提示在SLE活动期B细胞高度激活、增殖并大量表达CD23,且该种表达与ANA、抗dsDNA抗体产生水平无直接关系.

关键词: 红斑狼疮, 系统性, CD23

Abstract: In order to study the relationship between expression of CD23 on B cells and the deve lopment of SLE,and possible role in the pathogenesis of SLE,we used ABC immunohistochemistry and Northern dot blot hybridization technique to detect CD23 protein and mRNA expression on PBMC in SLE patients and healthy controls.The results showed:CD23 protein and mRNA expression was significantly higher in active SLE patients than those in controls(P<0 01),and showed positive linear correlation between CD23 expression and disease activity(rs=0 3814,P<0.05),but there was no significant difference between the patients after treatment in comparison with controls(P>0.05).There were no significant difference(P>0.05) in the expression of CD23 protein on PBMC among active SLE patients with different levels of ANA or dsDNA antibody,with or without renal or brain involvment as well as treated with corticosteroids and/or other immunosuppressants.It was implicated that abnormal activation and proliferation of B cells lead to over expression of CD23 protein and mRNA in active SLE,but there was not direct relationship between over expression of CD23 and the levels of ANA or anti dsDNA antibody.

Key words: Lupus erythematosus,Systemic, CD23, Expression