中华皮肤科杂志 ›› 1997, Vol. 30 ›› Issue (4): 232-234.

• 论著 • 上一篇    下一篇

以BP180NC16a融合蛋白检测大疱性类天疱疮抗体

林麟1, 桥本隆2, 靳培英1, 王峰来1   

  1. 1. 中国医学科学院, 中国协和医科大学皮肤病研究所, 南京 210042;
    2. 日本东京庆应大学医学部皮肤科
  • 收稿日期:1996-09-26 修回日期:1997-04-17 出版日期:1997-08-15 发布日期:1997-08-15
  • 基金资助:
    卫生部科研基金

Investigation of Bullous Pemphigoid Autoantibody with the NC16a Domain Fusion Protein of BP180 by Immunoblotting

Lin Lin1, T. Hashimoto2, Jin Peiying1   

  1. Institute of Dermatology, Peking Union Medical College, Chinese Academy of Medical Sciences, Nanjing 210042
  • Received:1996-09-26 Revised:1997-04-17 Online:1997-08-15 Published:1997-08-15

PDF (PC)

246

赞

摘要： 为了研究大疱性类天疱疮(BP)的BP180抗原上的抗体结合表位,了解BP血清中抗体与BP180NC16a融合蛋白反应的关系,进一步探讨分子生物学技术运用于表皮下大疱性皮肤病的病因分析和临床诊断,用基因工程技术,制备原核细胞内表达的完整性BP180NC16a区段融合蛋白,以此为抗原用免疫印迹法检测了64例BP血清、2例瘢痕性类天疱疮(CP)血清。结果在64例BP血清中48例阳性(75.0%),用人表皮提取蛋白抗原时43例阳性,阳性率67.2%,两者无统计学差异。2例CP血清均阳性。研究表明完整性BP180NC16a区段包含了大部分BP180抗体结合表位,是BP180中重要的抗原部分。CP抗体与BP抗体有共同性抗原结合点。虽然BP180NC16a融合蛋白技术在临床诊断运用中有一定的局限性,但已表现出明显的实用价值。

关键词: 类天疱疮, 融合蛋白, 免疫印迹

Abstract: In order to study the antigenic epitope on the BP180 and the relationship between the autoantibodies in bullous pemphigoid (BP) sera and BP180 NC16a fusion protein, we examined sera from 64 BP and 2 cicatricial pemphigoid (CP) patients using immunoblotting technique with the fusion protein covering the entire BP180 NC16a domain expressed by prokaryotic bacteria. The results showed that 48 of the 64 BP sera (75.0%) reacted with this fusion protein, which was higher than that with normal human epidermal extracts (43 positive, 67.2%). However, the difference between them was not statistically significant. All 9 CP sera also recognized the fusion protein. The results also indicated that the entire BP180 NC16a domain contains most epitopes of BP180 autoantibody, which represents the important part of BP180 autoantigen. The antibodies of BP and CP share a common antigenie site. Although there are now some limitations of using BP180 NC16a fusion protein in clinical diagnosis, it might be of use in the study of the pathogenesis and differential diagnosis of bullous diseases.

Key words: Pemphigoid, Fusion protein, Immunoblotting