中华皮肤科杂志 ›› 1996, Vol. 29 ›› Issue (6): 427-429.

• 论著 • 上一篇    下一篇

新型免疫抑制剂FK506对小鼠树枝状表皮T细胞的影响

米庆胜1, 王雅坤3, 陈洪铎3, 顾军4, 周丽2   

  1. 1. 山东省泰山医学院皮肤性病科 271000;;
    2. 山东省泰山医学院生理教研室
    3. 沈阳中国医科大学第一医院皮肤科;
    4. 上海第二军医大学长海医院皮肤科
  • 收稿日期:1995-10-09 修回日期:1996-01-24 出版日期:1996-12-15 发布日期:1996-12-15
  • 基金资助:
    山东省自然科学基金,中华医学会皮肤科学会研究基金

Efects of Novel Immunosuppressant FK506 on Dendritic Epidermal T Cells of Mice

Mi Qingsheng1, Wang Yakun3, Chen Hongduo3   

  1. Department of Dermatovenereology, Taishan Medical Colege, Taian 271000
  • Received:1995-10-09 Revised:1996-01-24 Online:1996-12-15 Published:1996-12-15

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摘要： 为了探讨新型免疫抑制剂FK506对小鼠树枝状表皮T细胞(DETC)的影响,我们应用抗Thy-1单克隆抗体间接免疫荧光技术动态观察了体内外应用FK506后小鼠DETC密度的变化.结果表明:①腹腔注射FK5060.32mg·kg·d及0.96mg·kg·d2～7天后DETC密度明显下降(P<0.05,P<0.01),且呈一定的量效关系.②1μg/ml及10μg/mlFK506体外浸育皮片2个小时及10个小时后,DETC密度亦明显下降(P<0.05,P<0.01).提示FK506对小鼠的免疫抑制作用至少部分可能与改变DETC功能相关.

关键词: FK506, 树枝状表皮T细胞

Abstract: In order to determine theefect of novel immunosuppressant-FK506 on dendritic epidermal Tcells(DETC)of mice,we sequentially investigated the density of DETC after the administration of FK506 in vitro and vivo,using indirect immunofluorescencete chnique with anti-Thy-1 McAb.The results showed that:(1)The density of DETC significantly decreased in a dose-dependent manner 2～7 days after intra-peritoneal injection with FK506 at 0.32mg·kg·d and 0.96mg·kg·drespectively (P<0.05,P<0.01);(2)The density of DETC in skin graft of mice decreased 2hours and 10 hours after treatment with FK506 at 1μg/ml and 10μg/ml respectively(P<0.05,P<0.01).It suggested that the immunosuppressiveaction of FK506 in miceskinmay,at least in part,be related to the change of the function of DETC.

Key words: FK506, Tcels, dendritic epidermal