中华皮肤科杂志 ›› 1994, Vol. 27 ›› Issue (5): 273-275,328.

• 论著 • 上一篇    下一篇

聚合酶链反应对53例皮肤结核病和结节性皮肤病的研究

冉玉平1, 周光平1, 步宏2, 杨秀英2   

  1. 1. 华西医科大学附属第一医院皮肤科, 成都610041;
    2. 华西医科大学附属第一医院病理科
  • 收稿日期:1993-10-18 修回日期:1994-05-16 出版日期:1994-10-15 发布日期:1994-10-15
  • 基金资助:
    四川省卫生厅科研基金

Study of 53 Cases of Cutaueous Tuberculosis and Nodular Dermatoses by Polymerase Chain Reaction

RAN Yu-Ping1, ZHOU Guang-Ping1, BU Hong2   

  1. Derpartment of Dermatology, The First Affiliated Hospital, West China University of Medical Sciences, Chengdu 610041
  • Received:1993-10-18 Revised:1994-05-16 Online:1994-10-15 Published:1994-10-15

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摘要： 采用聚合酶链反应(PCR)从临床诊断或病理诊断为皮肤结核病(CT)或结节性皮肤病(NDs)的53份石蜡包埋组织中检测结核杆菌DNA.PCR阳性率在临床诊断为CT和NDs者中分别为45%和47%.PCR与临床诊断的一致率为47%.PCR阳性率在病理诊断为CT和NDs者中分别为42%和53%.PCR与病理诊断的一致率为43%.以上结果提示皮肤结核病和结节性皮肤病在临床和病理上都不易区别,辅以PCR技术有助于从皮损中找到结核杆菌感染的病原学证据。

关键词: 结核, 皮肤, 结节性皮肤病, 聚合酶链反应

Abstract: Mycobacterium tuberculosis DNA was detected by polymerase chain reaction(PCR) from 53 samples of paraffin embedded tissues which were clinically or pathologically diagnosed as cutaneous tuberculosis (CT) or nodular dermatoses (NDs). The positive identification rates by PCR in clinically diagnosed CT and NDs patients were 45% and 47%, respectively. The consistency in diagnosis between PCR and the clinical diagnosis was 47%. The positive identification rates by PCR in pathologically diagnosed CT and NDs patinets were 42% and 53%,respectively. The consistency in diagnosis between PCR and the pathological diagnosis was 43%. These results indicate that it is difficult to successfully differentiate between cutaneous tuberculosis and nodular dermatoses either clinically or pathologically, and that the PCRmethod is useful to detect pathogenic evidence in the lesions of tuberculous infection.

Key words: Tuberculosis,cutaneous, Nodular dermatoses, Polymerase chain reaction