中华皮肤科杂志 ›› 1992, Vol. 25 ›› Issue (5): 304-306.

• 论著 • 上一篇    下一篇

人角朊细胞血小板活化因子的生物合成

刘宝军1, 郑茂荣1, 张素珍2, 戴方平2   

  1. 1. 第二军医大学长海医院皮肤科, 上海, 200433;
    2. 第二军医大学烧伤研究
  • 收稿日期:1991-05-07 修回日期:1991-10-04 出版日期:1992-10-15 发布日期:1992-10-15

Biosynthesis of Platelet Activating Factor by Human Skin Keratinocytes in Vitro

LIU Bao-Jun1, ZHENG Mao-Rong1   

  1. Changhai Hospital, The Second Military Medical University, Shanghai 200433
  • Received:1991-05-07 Revised:1991-10-04 Online:1992-10-15 Published:1992-10-15

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摘要： 用原代培养角朊细胞,经钙离子载体A23187刺激,能合成和释放一种使阿斯匹林处理洗涤兔血小板发生聚巢的物质,经4种方法证明为血小板活化因子(PAF).A23187刺激角朊细胞PAF的生成具有时间和剂量依赖关系.外源性乙既辅醉A、花生四烯酸能增加PAF的生成.本文证明了正常人皮肤角朊细胞能合成和释放PAF.

关键词: 血小板激活因子, 角朊细胞

Abstract: The synthesis and release of PAF by keratinocytes from normal human skin was investigated in vitro.When keratinotytes in suspension (1×108 cells) were stimulated with 2M Ca++ ionophore A23187,they synthesized a material that aggregated aspirin-treated washed rabbit platelet and way identified as PAF by four methods.

Key words: Platclct activating factor, Kerstinocytes