中华皮肤科杂志 ›› 1990, Vol. 23 ›› Issue (5): 316-318.

• 论著 • 上一篇    下一篇

雷公藤T4单体的免疫调节作用 Ⅱ. T4影响IVK细胞活性的机理探讨

骆丹1, 徐文严2   

  1. 1. 南京铁道医学院附院皮肤科;
    2. 中国医学科学院皮肤病研究所
  • 收稿日期:1989-05-13 修回日期:1989-11-16 发布日期:1990-10-15

Immunoregulatory of effects of monomer T4 from tripterygium Wilfordii Hook Ⅱ. The effect of T4 on NK activity of mouse splenocytes

LUO Dan1, XU Wen-Yan2   

  1. Institete of Dermatology, Chinese Academy of Medical Sciences, Nanjing
  • Received:1989-05-13 Revised:1989-11-16 Published:1990-10-15

PDF

439

赞

摘要： 本文从反映NK细胞活性效应的内在因素着手,以细胞毒动力学及单细胞法观察T4单体影响NK细胞的最大杀伤速率(Vmax),参加杀伤反应的效应细胞频率(%E)以及效靶细胞间亲和性的改变.结果表明:小剂量T4增大Vmax及%E,并使每个效应细胞的杀伤能力(k2)增强,较大剂量T4抑制Vmax,%E及k2.体外单细胞法证实:0.01μg/mlT4增加效靶细胞间的结合及杀伤,而1,0μg/mlT4并不明显影响效应细饱对靶细胞的结合,但使杀防频率降低显著.

Abstract: From the view of intrinsic mechanism of NK cytotoxicity, the kinetic model of cytotoxic reaction and tlse singly cell assay were used to observc and analyze the effecting link of T4 on NK activity. The results showed that the dosage enhancing NK activity could increase the frequency(%E) of killer cell in the splenocyte population, axed the rate constant of lysis(K2) so that the maximum (Vmax) of NK cell was raised.