Abstract: Objective To explore the effects of antisense NF-κB p65 oligodeoxynucleotides on UV-induced IL-6 secretion of keratinocytes. Methods NF-κB p65 oligodeoxynucleotides were transfected to a keratinocyte cell line, HaCaT cells, mediated by lipofectamine. The NF-κB p65 protein in the cells was measured by Western blot assay, the mRNA level of NF-κB p65 was detected by RT-PCR, and UV-induced IL-6 level was determined by ELISA pre-and post-transfection and/or UVB irradiation. Results The NF-κB p65 protein expression was significantly increased in UVB (10, 20, 30 mJ/cm²) irradiation groups as compared with that of the control groups (P<0.05). The p65 mRNA and UVB-induced IL-6 were inhibited after the keratinocytes were transfected with p65 antisense oligodeoxynucleotides at the concentrations of 2, 4 and 8 μg/mL (P<0.05). Conclusions UV-induced IL-6 secretion of keratinocytes might be mediated by NF-κB, and could be inhibited by NF-κB p65 antisense oligodeoxynucleotides.

Key words: Ultraviolet rays, Keratinocytes, NF-kappa B, Oligonucleotides, antisense