Abstract: ZHANG Xiao-li, GAO Lai-qiang, WU Xi-fu, ZHANG Hong *, ZHANG Ge-hua, YIN Xing-feng. *First Affiliated Hospital, Institute of Mycology, Jinan University, Guangzhou 510632, China Corresponding author： ZHANG Hong, Email: tzhang@jnu.edu.cn 【Abstract】 Objective To identify and analyze plasma membrane proteins differentially expressed between fluconazole-sensitive and -resistant C.albicans strains. Methods Two C.albicans strains from a same parent, including the fluconazole-sensitive C.albicans strain CA-3 and fluconazole-resistant C.albicans strain CA-16, served as the subject of this study. Plasma membrane proteins were isolated from both of the C.albicans strains, and subjected to two-dimensional polyacrylamide gel electrophoresis analysis for the screening of differentially expressed proteins, which were then identified by using matrix assisted laser desorption/ionization time-of-flight mass spectrometry. The resultant data were searched against a protein database for C.albicans. Results Twenty-two proteins were identified to be differentially expressed between the fluconazole-resistant and -sensitive C.albicans strain. Of them, 6 proteins（Adh1p, Csp37p, Pgk1p, Pgk1p and 2 unnamed proteins, i.e., gi227305312 and gi53954641） were highly expressed, while 16 proteins （Aco1p, Aco1p, Hsp78p, Gut2p, Sdh12p, Ilv2p, Ndh51p, Ndh51p, Atp1p, Pda1p, Srb1p, Idh1p, Tdh1p, Cyt1p, Cox4p, Cox13p） were lowly expressed in the fluconazole-resistant C.albicans strain compared with the fluconazole-sensitive strain. Conclusion The plasma membrane proteins differentially expressed between fluconazole-sensitive and -resistant C.albicans strain are mainly implicated in energy metabolism and mitochondrial function. 【Key words】 Candida albicans; Fluconazole; Drug resistance, fungal; Membrane proteins; Proteomics

Key words: Membrane protein, proteomics