Abstract: In order to confirm the specific binding of anti-Fos RI InAb to the surface of LC, IEM using immunogold labeling was performed. We found that LC were anti-Fos RI CAb reactive epidermal cells as evidenced by the discontinuous distribution of sum gold particles along the surface of LC, but no other epidermal cells. Furthermore, in an attempt to search for Fos RI gene expression by LC, PCR experiment was performed and the result revealed the presence of Fos RI al 0 and Y chain transcripts in LC-enriched, but not in LC-depleted, cpidermal cell suspensions. Collectively the present studies Provide direct evidence for the presence of Fos RI on epidcrmal LC.

Key words: Langerhans cells, Receptor,IgE-Fc