聚合酶链反应检测生殖器疣与癌中人乳头瘤病毒

Abstract

Abstract: A newly developed general primer-mediated polymerase chain reaction technique (PCR) was used to analyze human papillomavirus (HPV) in 177 specimens of genital warts and cancers.The results:HPV DNA sequences were found in 98% (98/100) genital warts,83% (10/12) PIN M,80% (12/15) CIN Ⅲ 80% (16/20) penile squamous cell carcinoma (SCC) and 90% (27/30) cervical SCC' Of the cases that contain viral DNA,Ⅲ HPV-6,11 were detected in 95% (93/98) genital warts,and HPV-16 in 88% (38/43) genital SCC.These results indicate:(l) PCR is a useful approacll for detecting HPV in dysplastic and SCC suspected of having an HPV aetiology,(2) In Chongqing area,HPV-6,11 and HPV 16 are the prevalent HPV types iii benign and malignant genital lesions respectively

Key words: Human papillomavirus, Polymerase chain reaction

LIU Yuan-Lin, LIU Rong-Qing, CHEN Hong-Shu. Detection and Typing of Human Papillomavirus in Biopsy Specimens of Genital Warts and Cancers Using the Polymerase Chain Reaction Technique[J].Chinese Journal of Dermatology, 1994, 27(3): 148-149,189.

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Detection and Typing of Human Papillomavirus in Biopsy Specimens of Genital Warts and Cancers Using the Polymerase Chain Reaction Technique

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