Abstract: Antibody bridging keratinocyte/mononuclear leucocyte adhesion(ABKMA), an experimental model in vitro for psoriasis, has been used in the investigation of cytokine network changes. ABKMA was formed by using normal human epidermal cells incubated with IgG of patients with psoriasis, and then mononuclear leucocytes(ML) of patients with psoriasis were added. Interleukin 2 and interleukin 6 in culture supernate were estimated by ABC amplified ELISA. The results indicated that the synthesis and secretion of interleukin 2 and interleukin 6 were higher than normal IgG and serum free medium controls. Immunohistochemistry staining was used to demonstrate the cell surface interleukin 2 receptor α chain (IL2Rα). The expression of IL2Rα⁺ML/ML and IL2Rα⁺ML/adhesion ML was 4.6% and 40.4% respectively. Both these data were higher than corresponding controls.

Key words: Psoriasis, Interleukin 2, Interleukin 6