Abstract: We analysed the expression of the BPAG2 recombinant cDNA clone to obtain the purified BPAG2 fragment in vitro.The nonfused fragment of 22.5 kD was expressed by temperature induction after the pKPL-BPAG2 transformed into bacteria POP2136.This expression band was correspondent to its cDNA clone.The product accounted for 18.1% of total bact erial proteins.The recombinant fragment was purified by SDS-PAGE and ident ified that it maintained specific immunological features of BPAG2.The seresult smight be used as an in vitro diagnostic test of BP.

Key words: Pemphigoid, bulous, Antigen, cDNA