重组腺病毒介导金黄色葡萄球菌肠毒素A基因在小鼠B16黑素瘤细胞中的表达

Abstract

Abstract: Objective To investigate staphylococcal enterotoxin A (SE A) gene expression on murine B16 melanoma cells mediated by replication-defective recombinant adenovirus. Methods SEA expression on the B16 melanoma cells was detected by reverse transcription-polymerase chain reaction (RT-PCR) and flow cytometry (FCM). Results RT-PCR and FCM proved that SEA could specifically express on the B16 melanoma cells. Conclusion The successful expression of SEA gene on the B16 melanoma cells might be used for the targeted treatment of mali gnant melanoma.

Key words: Gene expression, Melanoma, experimental, Enterotoxins

MU Xia, LU Hong-guang, YU De-hou, WU Cheng-long, WANG Yu. The Expression of Staphylococcal Enterotoxin A(SEA) Gene on Murine B16 Melanoma Cells Mediated by Replication-defective Recombinant Adenovirus Vector[J].Chinese Journal of Dermatology, 2005, 38(6): 365-367.

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The Expression of Staphylococcal Enterotoxin A(SEA) Gene on Murine B16 Melanoma Cells Mediated by Replication-defective Recombinant Adenovirus Vector

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