Abstract: For more accurate and simple identification of Fonsecaea pedrosoi based on genomic composition in clinical and experimental mycological laboratories,we report the first construction of F.pedrosoi genomic DNA library in plasmid pUC18 vector by using the insertion of Sau 3AI partially digested fragments 2.5 to 6.0kb in length.The exact probability of having any DNA sequence represented in the library is above 84%.It is suggested that the library will be of value for the identification and mycological diagnosis of F.pedrosoi infection in the future,and be useful as the basis for screening a series of genomic DNA probes.

Key words: Fonsecaea pedrosoi, DNA library