Abstract: The most commonly used method to obtain the human scalp anagen hair dermal papillae is the microdissection on excised tissue. This method have many drawbacks, such as laboriousness, low efficiency, uneasy adhesion, and poor growth. Here we report a more efficient method for the isolation of dermal papillae from human scalp hair follicles. The dermal sheath of lower part of hair follicles was entirely digested by collagenase D into isolated dermal sheath cells and dermal papillae. The advantages of the modified method are: microdissection can be omitted, work load is reduced, it is more efficient, and shows considerable improvement on the cell adhesion and growth. Futhermore, the dermal papillae can be isolated on a large scale and remained intact. The dermal papilla cells showed positive stain with alcian blue, PAS and toluidine blue, which implies that the dermal papilla cells is a highly specialized fibroblasts.

Key words: Hair follicle, Cells,cultured