Abstract: Objective In order to study the pathogenesis of human papillomavirus(HPV) and seek for a therapeutic approach of the diseases caused by HPV,the construction of HPV18 E6E7 antisense RNA expressing recombinants was studied.Methods We amplified the HPV18 E6E7 816bp by PCR with HPV18 plasmid DNA as the template.pLNSX retroviruses were used as vectors,the HPV18 E6E7 retrovirus recombinants were constructed.And then the recombinants were cleaved with restriction endonuclease and hybridized with Southern blot for identifying the inserting direction and special check respectively.Results and conclusion The HPV18 E6E7 antisense RNA retrovirus expressing recombinants were screened and obtained,which had laid the foundation of studying the function of E6E7 genes further and explore whether the antisense technique can adjust and control the expression of E6E7 genes.

Key words: HPV18E6E7, Retrovirus vector, Antisense RNA