培养人角朊细胞中白介素15的基因表达

Abstract

Abstract: Objective To study the expression and regulation of interleukin-15 (IL-15) in epidermal keratinocytes. Methods The expression of IL-15 mRNA in cultured normal human keratinocytes (NHKs) and human squamous cell carcinoma cell line (HSC-5) was analysed, and the effect of dexamethasone on IL-15 mRNA expression was studied by using RT-PCR and Northern blotting technique. Results The results showed that both NHKs and HSC-5 expressed IL-15 constitutively. The level of IL-15 mRNA was significantly decreased after the cells were cultured with 10^-6M dexamethasone. Conclusion It is suggested that keratinocyte-derived IL-15 might be involved in the development of certain inflammatory skin diseases.

Key words: Keratinocytes, Interleukin-15, Blotting,Northern

Han Gangwen, Keiji Iwatsuki, Ma Shengqing. The Expression of IL-15 mRNA in Cultured Human Keratinocytes[J].Chinese Journal of Dermatology, 1998, 31(4): 236-238.

References

1 Grabstein KH, Eisenman J, Shanebeck K, et al. Cloning of a T cell growth factor that interacts with the β chain of the interleukin-2 receptor. Science, 1994,264:965-968.
2 Bamford RN, Battiata AP, Borton JD, et al. Interleukin (IL)15/IL-T productionby the adult T-cell leukemia cell line HuT-102 is associated with a human T-cell lymphotrophic virus type ⅠR region/IL-15 fusion message that lacks many upstream AUGs that normally attenuate IL-15 mRNA translation. Proc Natl Acad SciUSA, 1996,93:2897-2902.
3 Carson WE, Giri JG, Lindemann MJ, et al. Interleukin (IL)15 isa novel cytokinethat activates human natural killer cells via components of the IL-2 receptor.J Exp Med, 1994,180:1395-1403.
4 Lewko WM, Smith TL, Bowman DJ, et al. Interleukin-15 and the growth oftumor-dervied activated T-cells. Cancer Biother, 1995,10:13-20.
5 Armitage RJ, MacDuff BM, Eisenman J, et al. IL-15 has stimulatory activity forthe induction of B cell proliferation and differentiation. J Immunol, 1995,154:483-490.
6 Mohamadzadeh M, Takashima A, Dougherty I, et al. Ultraviolet Bradiation up-regulates the expression of IL-15 in human skin. J Immunol, 1995,155:4492-4496.
7 韩钢文, 周立军, 马圣清, 等. 聚合酶链反应Dig-dUTP掺入法制备桥粒芯蛋白1、3型探针. 中华皮肤科杂志, 1997,30:248-250.
8 Blauvelt A, Asada H, Klaus-Kovtun V, et al. Interleukin-15 mRNA is expressedby human keratinocytes, Langerhans cells, and blood-derived dendritic cells andis downregulated by ultraviolet B radiation. J Invest Dermatol, 1996,106:1047-1052.
9 Sorel M, Cherel M, Dreno B, et al. Production of interleukin-15 by human keratinocytes. Eur J Dermatol, 1996,6:209-212.
10 McInnes IB, Al-Mughales J, Field M, et al. The role of interleukin-15 in T-cell migration and activation in rheumatoid arthritis. Nat Med, 1996,2:175-182.

[1]	LI Wei, HAN Hua, XING Ying, KUANG Ying, MA Sun-kai, FU Meng, WANG Gang, LI Cheng-xin, LIU Yu-feng. Construction of a Transgenic Mice Model with High Serum Titer of Natural Anti-keratin Autoantibody[J]. Chinese Journal of Dermatology, 2005, 38(12): 759 -761 .
[2]	马国安, 王宝娟, 张莉, 李晓燕. [J]. Chinese Journal of Dermatology, 2005, 38(12): 758 .
[3]	. [J]. Chinese Journal of Dermatology, 2005, 38(11): 701 -702 .
[4]	. [J]. Chinese Journal of Dermatology, 2005, 38(11): 702 -703 .
[5]	. [J]. Chinese Journal of Dermatology, 2005, 38(9): 545 .
[6]	刘晓明, 张振颖, 金礼吉, 杨国玲, 王华, 林熙然, 安利佳. [J]. Chinese Journal of Dermatology, 2005, 38(8): 518 -519 .
[7]	FU Jun-fen, LIANG Li, DONG Guan-ping, WANG Xiu-min, ZHENG Min. Benign Acanthosis Nigricans in Obese Children:An Indicator for High Risk of Diabetes[J]. Chinese Journal of Dermatology, 2005, 38(7): 429 -431 .
[8]	邓丹琪, 韩云涛, 陈浩, 李海英. [J]. Chinese Journal of Dermatology, 2005, 38(7): 451 -452 .
[9]	. [J]. Chinese Journal of Dermatology, 2005, 38(7): 461 .
[10]	XU Shao-bin, TAO Yu-fen, CHU Zheng-tao, HUANG Xiao-qin, BAN Gui-hong, YU Jian-kun, CHU Jia-you. Association of HLA-A Alleles with Systemic Lupus Erythematosus[J]. Chinese Journal of Dermatology, 2005, 38(1): 3 -4 .

[1]	Zhang Yuanyuan, Zeng Yue, Zhu Yanxia, Lian Cuihong. Effect of exosomes from human adipose-derived stem cells on wound healing and migration potential of HaCaT cells: a preliminary experimental study [J]. Chinese Journal of Dermatology, 2019, 52(9): 616-623.
[2]	Li Lingjia, Liu Tongyun . Expression of caspase-14 in skin lesions of patients with chronic actinic dermatitis and effect of ultraviolet B radiation on its mRNA and protein expression in HaCaT cells [J]. Chinese Journal of Dermatology, 2019, 52(7): 486-490.
[3]	Chen Yiwen, Su Ting, Su Zhonglan. Association between psoriasis and STAT3 [J]. Chinese Journal of Dermatology, 2019, 52(7): 502-505.
[4]	Shou Yanhong, Zhang Zhen, Luo Xiaoqun, Chen Sheng′an, Li Feng, Zhu Xiaohua, Lin Jinran, Qin Haihong, Du Juan, Chen Sunyi, Yang Yongsheng, Xu Jinhua . Effect of interferon-γ combined with tumor necrosis factor-related apoptosis-inducing ligand on programmed necrosis of HaCaT cells and its mechanisms [J]. Chinese Journal of Dermatology, 2019, 52(5): 302-309.
[5]	Zhou Wenjiao, Ren Jie, Han Long, Liu Xinxin, Tang Kunlong, Luo Suju. Effects of interleukin-22 on the of CC chemokine ligand 27 in human epidermal keratinocytes [J]. Chinese Journal of Dermatology, 2018, 51(8): 592-594.
[6]	Xiao-Jie SONG. Construction and characterization of a full-thickness skin model with collagen matrix [J]. Chinese Journal of Dermatology, 2018, 51(7): 490-494.
[7]	. Effects of camptothecin on autophagy of human primary keratinocytes [J]. Chinese Journal of Dermatology, 2018, 51(7): 510-514.
[8]	Yue-ping ZENG Cheng Chen. Effect of microRNA-143 on interleukin-13-induced of kallikrein 7 in human epidermal keratinocytes [J]. Chinese Journal of Dermatology, 2018, 51(4): 256-259.
[9]	Ying-Di LIU Bingxue BAI juan liangma. Protective effect of exogenous biliverdin against ultraviolet B-induced photodamage in HaCaT cells [J]. Chinese Journal of Dermatology, 2018, 51(4): 279-282.
[10]	Ting-ting JIN Erwin Tschachler Yi ZHAO. Quantitative analysis of autophagy in green fluorescent protein-light chain 3 transgenic murine keratinocytes [J]. Chinese Journal of Dermatology, 2018, 51(3): 182-185.
[11]	Zheng Yuxin, Min Min, Zhu Ziyu, Yang Xiaohong, Cao Yi, Zheng Min. Effects of glycyrrhetinic acid on proliferation and apoptosis of, as well as miR-21 xpression in keratinocytes from patients with psoriasis [J]. Chinese Journal of Dermatology, 2018, 51(11): 802-805.
[12]	. Reconstruction of tissue-engineered skin using keratinocyte serum-free medium [J]. Chinese Journal of Dermatology, 2016, 49(2): 103-107.
[13]	. Expression of aryl hydrocarbon receptor in human epidermis, hair follicles and sebaceous glands and its significance [J]. Chinese Journal of Dermatology, 2011, 44(11): 761-764.
[14]	. Activation of the Akt/mTOR pathway confers protection against UVB-induced apoptosis in HaCaT cells [J]. Chinese Journal of Dermatology, 2010, 43(9): 633-636.
[15]	. Influences of glucocorticoids with different potency on the expression of glucocorticoid receptors on HaCaT cells: a comparative study [J]. Chinese Journal of Dermatology, 2010, 43(6): 424-425.

The Expression of IL-15 mRNA in Cultured Human Keratinocytes

PDF

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 10