Abstract: Objective To investigate the feasibility of transient transfection of human VEGF₁₆₅ gene into HaCaT cells and the effects of suPernatant from transfected cell culture on pig hair follicles in vitro.Methods PIRES₂-EGFP-VEGF₁₆₅ was transiently transfected into HaCaT cells with lipofectaminem^TM 2000. Expression of EGFP (enhanced green fluorescence protein,EGFP) was observed by laser confocal microscopy.VEGF in the supernatant was detected by ELISA.Further,the supernatant was added to pig hair follicles cultured in vitro.The growth and morphologic changes of hair follicles were measured.Results PIRES₂-EGFP-VECF₁₆₅ was successfully transfected into HaCaT cells,which confirmed by laser confocal microscopy and ELISA.Moreover,the supernatant of HaCaT cells transfected with PIRES₂-EGFP-VEGF₁₆₅ could accelerate the growth of Pig hair follicles and Prolong their anagen phase.Conclusion PIRES₂-EGFP-VEGF₁₆₅ could be successfully transiently transfected into HaCaT cells,and expressed VEGF could upregulate the growth of pig hair follicles invitro.

Key words: Endothelial grwth factors, Enhanced green fluorescent protein, Hair follicle, Cells,cultured