Abstract: Objective To investigate the role of protein kinase Cζ(PKCζ)in the proliferation of keratinocytes. Methods By gene transfection, the influence of the expression of antisense PKCζon the proliferation of keratinocytic cell line-Colo16 was studied. Results The morphology of the keratinocytes expressing antisense PKCζwas changed, the growth rate of the keratinocytes reduced significantly, and they were inhibited in G1 phase. Conclusion The expression of antisense PKCζinhibits the proliferation of Colo16 keratinocytes.

Key words: Protein kinase C, Keratinocytes