淋球菌染色体mtrR基因突变对淋球菌多重耐药性的影响

Abstract

Abstract: Objective To investigate the mechanism of multiple antibiotic resistance of Neisseria Gonorrhoeae chromosome.Methods The mtrR gene sequence of Neisseria Gonorrhoeae was examined by polymerase chain reaction and Sanger′s method, the drug susceptibility testing of relevant strains was done.Results Among 37 stains resistant to penicillin, 21 were resistant to tetracycline, erythromycin and Triton X-100 simultaneouely; one of them was resistant to spectinomycin as well; 5 strains were resistant to erythromycin only. All penicillin-resistant strains had a single base mutation in mtrR gene, and spectinomycin-resistant strain mutated in two position of mtrR gene.Conclusion The missense and null mutations in the mtrR gene of Neisseria Gonorrhoeae resulted in the emergence of multiple antibiotic resistance.

Key words: Neisseria Gonorrhoeae, genes, mtrR, Microbial sensitivity tests

PAN Weihua, LIAO Wanqing, LI Zhigang. Effect of mtrR Gene Mutation on Multiple Antibiotic Resistance of Neisseria Gonorrhoeae[J].Chinese Journal of Dermatology, 1999, 32(3): 152-154.

[1]	SHEN Hong, TANG Xu, WANG Yi-ling, XU Ai-e. Expression of p73 in Benign and Malignant Epidermal Tumors and in Normal Skins[J]. Chinese Journal of Dermatology, 2005, 38(12): 741 -744 .
[2]	GAO Ying, LI Chun-ying, MA Cui-ling, YIN He-hui, GAO Tian-wen. Expression of Double-stranded RNA-dependent Protein Kinase in Malignant Melanoma and Ordinary Nevi[J]. Chinese Journal of Dermatology, 2005, 38(12): 748 -750 .
[3]	XU Yan, CHENG Hao, ZHAO Ke-jia, YE Jun, ZHANG Xing, SONG Tao, CHEN Min-lI, WANG Qi. The Specific Immune Response Elicited by Recombinant CRT120/HPV6bE7 DNA Vaccine in Mice[J]. Chinese Journal of Dermatology, 2005, 38(12): 755 -758 .
[4]	. [J]. Chinese Journal of Dermatology, 2005, 38(12): 762 -763 .
[5]	廖列辉, 张锡宝, 范瑞强, 刘文静. [J]. Chinese Journal of Dermatology, 2005, 38(12): 761 .
[6]	. [J]. Chinese Journal of Dermatology, 2005, 38(12): 777 -778 .
[7]	. [J]. Chinese Journal of Dermatology, 2005, 38(11): 697 -698 .
[8]	房婕, 韩德平, 顾军, 郭峰, 花美仙. [J]. Chinese Journal of Dermatology, 2005, 38(11): 699 -700 .
[9]	. [J]. Chinese Journal of Dermatology, 2005, 38(11): 700 .
[10]	LI Xiao-jie, WANG Hong-sheng, WU Qin-xue, CUI Pan-gen, LIU Xun-quan. Development of PCR-RFLP for Identification of Eight Mycobacteriai Species[J]. Chinese Journal of Dermatology, 2005, 38(9): 533 -535 .

[1]	Luo Qiuhong, Pan Kaisu, Luo Hong, Cao Cunwei. Evaluation of in vitro antifungal activity of osthole against Talaromyces marneffei in yeast phase [J]. Chinese Journal of Dermatology, 2019, 52(4): 262-265.
[2]	Li JIANG Chun-Yan GONG. Evaluation of in vitro antibacterial activity of 12 antibacterial agents against Propionibacterium acnes [J]. Chinese Journal of Dermatology, 2018, 51(6): 453-455.
[3]	Sun Mingyan, Wu Qianqian, Wang Yexin, Wang Nan, Liu Yanxia, Tao Yuanyong. Detection of 16S rRNA gene and biological characteristics of Mycobacterium abscessus [J]. Chinese Journal of Dermatology, 2018, 51(12): 901-904.
[4]	. In vitro antibacterial activity of fusidic acid cream against Staphylococcus epipermidis and Propionibacterium acnes isolates from acne vulgaris lesions [J]. Chinese Journal of Dermatology, 2016, 49(9): 658-659.
[5]	. In vitro evaluation of antibacterial activity of fusidic acid against Propionbacterium acnes [J]. Chinese Journal of Dermatology, 2014, 47(11): 823-825.
[6]	JIANG Fa-xing, Qi Mu-ge, DAI Xiu-qin, SUN Hou-hua, YE Shun-zhang, SU Xiao-hong. Molecular epidemiology of tetM gene in high-level tetracycline-resistant Neisseria gonorrhoeae isolates in Nanjing, 1999-2004 [J]. Chinese Journal of Dermatology, 2007, 40(3): 133-135.
[7]	ZHENG Bo, ZHONG Ming-ying, ZHANG Jin-ping, WANG Qian-qiu. In vitro antiviral susceptibilities of HSV-2 clinical isolates to acyclovir and valaciclovir [J]. Chinese Journal of Dermatology, 2007, 40(3): 143-145.
[8]	XU Yong-hao, LI Chun-yang, CHEN La-mei, HU Qin-feng. Correlation between antifungal susceptibility of clinical isolates of Candida albicans to fluconazole and ERG11 mutations [J]. Chinese Journal of Dermatology, 2007, 40(12): 756-758.
[9]	QI Mu-ge, JIANG Fa-xing, DAI Xiu-qin, SUN Hou-hua, YE Shun-zhang, WANG Qian-qiu, SU Xiao-hong. Ciprofloxacin resistance in Neisseria gonorrhoeae in Nanjing City,1999-2006 [J]. Chinese Journal of Dermatology, 2007, 40(12): 720-721.
[10]	LI Feng-xia, ZHANG Hong. In vitro study of the synergistic effect of tetrandrine and fluconazole against Candida albicans [J]. Chinese Journal of Dermatology, 2006, 39(8): 454-456.
[11]	HAN Jian-de, CHEN Mu-kai, LIAO Qi-man, WANG Li-hong, LING Hong-zhong, TU Yu-ying. In vitro susceptibility of genital Chlamychia trachomatis to several antimicrobial agents [J]. Chinese Journal of Dermatology, 2006, 39(10): 559-561.
[12]	LEI Xia, WU Jin-jin, ZHU Tang-you, LU Yuan-gang. Study on Antibacterial and Antifungal Effect of Composite Chitosan Dermal Substitute in vitro [J]. Chinese Journal of Dermatology, 2005, 38(4): 220-221.
[13]	ZHOU Wen-ming , ZHAO Jian-long, YANG Sen, CAO Hui-min, LI Wei, SHEN Yu-jun, ZHANG Shu-mei, DU Wen-hui, ZHANG Xue-jun. Detection of Pathogens of Urogenital Infections and Their Drug-resistant Types by a DNA Chip [J]. Chinese Journal of Dermatology, 2005, 38(2): 108-111.
[14]	LIN Neng-xing, ZHANG Li-xia, HUANG Chang-zheng, CHEN Hong-xiang, TU Ya-ting. The Mutation of IR Gene in the mtr System and Multiple Antibiotic Resistance of Neisseria gonorrhoeae [J]. Chinese Journal of Dermatology, 2005, 38(12): 719-721.
[15]	CHEN Hong-xiang, LIN Neng-xing, HUANG Chang-zheng, LI Jia-wen, LIU Hou-jun, TU Ya-ting. Construction of a Prokaryotic Expression Plasmid Encoding the mtrC Gene of N.gonorrhoeae and Its Expression in E.coli [J]. Chinese Journal of Dermatology, 2005, 38(11): 674-676.

Effect of mtrR Gene Mutation on Multiple Antibiotic Resistance of Neisseria Gonorrhoeae

PDF

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 10