Abstract:

Objective To study the DNA instability of the peripheral blood lymphocytes and T-cell line from skin lesions of mycosis fungoides(MF) and S閦ary's syndrome (SS).Methods The Comet assay,which was also called as SCGE (the single cell gel electrophoresis),was used to detecting the primary DNA damage at the level of single cell in specimens.The DNA damage of X-ray-irradiated peripheral blood lymphocytes and T lymphocytes from skin lesions,and its repair in lymphoma patients were studied,and compared with those in control persons.Results A significantly increased DNA instability in the majority of lymphocytes from the blood and skin lesions of MF and SS patients was observed,and reduced DNA repair of X-ray irradiated lymphocytes was found,when compared with the lymphocytes from healthy individuals.Conclusion The DNA stability and repairment of both blood and skin-homing lymphocytes from patients with MF and SS are significantly impaired,which suggests that MF and SS could develop from a clinically benign skin disease into an overt lymphoma.

Key words: Comet assay, Mycosis fungoides, Sezary’s Syndrome, DNA Instability