Abstract:

Objective To investigate the DNA polymorphism of F pedrosoi and to find the relationship between DNA patterns and morphology in different geographic areas Methods The total DNA was extracted with benzyl bromide Random amplification of polymorphic DNA(RAPD) assay was used to study the genetic similarity (GS) of 32 isolates from seven countries (15 isolates from Japan,3 isolates from China,4 from Venezuela,4 from Central America,2 from Columbia,2 from Mexico,1 from Brazil and 1 from Thailand) Results ①Of ten random primers used,three (primers 1,7,8) gave good reactions The sequences of them were:primer 1:5-GAGCCCTCCA-3,primer 7:5-CGGCCCCTGT-3,primer 8:5-AGGTCACTGA-3,②The RAPD patterns of 32 isolates showed some degrees of variation ③The isolates from the same country showed high genetic similarities ④15 isolates from Japan could be divided into 2 types by RAPD analysis with primer 8 ⑤With the same primer,the common DNA fragment could be gotten from more than 90%isolates Conclusion There are some genetic variations between 32 isolates of F-pedrosoi The genetic similarity of isolates is closely related to the geographic origin As a simple and rapid method,RAPD assay can be used for the study of epidemiology and genetic characterization of chromoblastomycosis

Key words: F-pedrosoi, Random amplification of polymorphic DNA assay, Genetic similarity