Abstract: Objective To investigate the changes of cell cycle related factors [PCNA,cyclinE,p21(WAF1/CIP1) and p27] in condyloma acuminatum(CA) and their significance.Methods HPV6/11 associated CA was diagnosed by in situ hybridization and the expression and distribution of PCNA,cyclinE,p21 and p27 in 50 HPV 6/11 positive CA specimens and 25 normal human skins (foreskins) were detected by SP immunohistochemistry technique.Results ① The expression of PCNA,cyclinE and p21 in skin lesions of CA was higher than that in normal epithelium,and there was no statistic difference for the expression of p27,and the distribution of these molecules was mainly in spinous and granular layers in CA.② There were correlations of different molecule expression in CA:between cyclinE and p21 and p27,between p21 and p27.Conclusion The data suggest that the expression of PCNA,cyclinE and p21 in HPV6/11 positive CA is increased and the coordination or restriction among PCNA,cyclinE,p21 and p27 might induce self-limited proliferation of kera-tinocytes.

Key words: Condyloma Acuminata, Proliferating cell nuclear antigen, Cyclins, Oncogene, protein p21, p27