Abstract: Objective To construct Herpes simplex virus-2 glycoprotein D polynucleotide vaccine,and to detect the glycoprotein D2(gD2)expression in guinea pig's tongue by intramuscular injection of gD2 polynucleotide vaccine. Methods The recombinant plasmids HSV-gD2 polynucleotide vaccine was constructed by molecular cloning technique. The guniea pigs were intramuscularly injected with 0.75% bupivacaine,after 7 days,they were divided into 3 groups(4 guinea pigs per group)and intramuscularly injected with 100μL 0.09% NaCl、pcDNA3、gD2-pcDNA3,three times at 7 day intervals respectively. All guinea pigs were sacrificed 2 days after the last injection,and the tongue,liver,kidney,spleen and quadricep muscle were removed for immunohistochemical assay (SP method). Results The recombinant pcDNA3-gD2 plasmid was constructed succuessfully. Immunohistochemical staining for gD2 was positive in the tongue injected with pcDNA3-gD2,but negative in the liver,kidney,spleen,heart and quadricep muscle. Conclusion Herpes simplex virus-2 glycoprotein D polynucleotide vaccine was constructed successfully,and gD₂ antigen can be expressed effectively in the tongue after injection of the HSV-gD2 polynucleotide vaccine in it.

Key words: Herpes,genitalis, Glycoprotein D, Polynucleotide vaccines