Abstract: Objective To construct antibody library in patient with pemphigus vulgaris using phage display antibody technique. Methods Total RNA was extracted from B cells of patients with pemphigus vulgaris(PV)using polymerase chain reaction,immunoglobulin VH and VL genes were amplified by specific primer of HuVHBACK,HuJHFOR,HuVkBACK and HuJkFOR. The VH and VL genes were then cloned into a phage vector and expressed on the phage surface as a fusion protein with product of geneⅢ. Results The antibody phage library of single chain antibodies of patients with PV was constructed. Conclusion We have obtained EC1-2 and EC3-4 purified protein of Dsg3 from constructed recombinant plasma in our laboratory which will facilitate the study on Dsg3 antibodies.

Key words: Pemphigus vulgaris, Phage display antibody technique, Antibody library