Abstract: Objective To investigate the efficacy,safety of allogeneic hematopoietic stem cell transplantation(Allo-HSCT)in lupus mice.Methods 22 BXSB was pre-treated with myleran and cyclophosphamide,12 BXSB were treated with CTX and prednisone as the control group.Peripheral blood stem cells of doner mouse(C57BL/6)after mobilizing with G-CSF were infused into recipients after pretreatment.Hematopoietic and immune reconstitution after transplantation were measured by blood cell analysis and flowcytometry.Recipients' renal pathological changes were measured with direct immunofluorescence after transplantation,proteinuria and anti-dsDNA antibody were also determined.Complications and mortality were compared between the two groups.Recipients'genetic map was analysed with PCR.Results Recipients'complete chimerism was observed after 60 days of transplantation.The lowest value of WBC declined to 0.2±0.1×10⁹/L in Allo-HSCT group.The duration of WBC increased from the lowest value up to 1.0×10⁹/L was47.5±12.8d in Allo-HSCT group.Early immune reconstitution could not be found in Allo-HSCT group.After transplantation reduction of proteinuria occurred in 95.5% BXSB,anti-ds-DNA antibody turned to negative in 54.5% BXSB,decrease of kindey immunofluorescence in90.9%BXSB,there was a significant difference between the two groups.There was no significant difference in the incidences of complication of bleeding,pneumonia and mortality between the Allo-HSCT group and the control group.Conclusions Allo-HSCT is an effective treatment method for lupus mice.It may be helpful in improving proteinuria and anti-dsDNA antibody and renal pathological changes of lupus mice.But the incidences of complication and mortality related to transplantation in Allo-HSCT group are higher than those in control group,so the value of Allo-HSCT in the treatment lupu mice has to be further studied.

Key words: Lupus erythematosus,systemic, Hematopoietic stem cell transplantation, Animals,experimental