Chinese Journal of Dermatology ›› 2003, Vol. 36 ›› Issue (2): 70-72.

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Analysis of T-cell receptorγgene rearrangement for cutaneous T-cell lymphomas

WANG Chen1,2, Sang Jian-li1, Zhang Hai-jiang1   

  1. College of Life Sciences, Beijing Normal University, Beijing 100875, China
  • Received:2002-02-19 Online:2003-02-15 Published:2003-02-15

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Abstract: Objective To study the early diagnosis of cutaneous T-cell lymphoma(CTCL).Methods DNA from39formalin-fixed,paraffin-embedded tissues of 24 cases of CTCL,2 cases of dubious lymphoma,and4cases with non-specific erythroderma,was amplified by polymerase chain reaction(PCR),with special primers of TCR Vγ1-8,Vγ9,and Jγ1/γ2 to analyze T-Cell receptorγgene clonal rearrangement(TCR-GR clonal).The specimens from30patients with non-specific dermatitis were taken as negative control.Results Clonal of TCR Vγ1-8 was demonstrated in38of39paraffin-embedded specimens.Vγ9-GR was shown in 37 of 39 specimens TCRγ-GR was shown especially in 13 patients with MF of early stage,2 patients with dubious CTCL,and4patients with non-specific erythroderma,as well as in 6 of 30 patients with histiologically nonspecific dermatitis which suggested that these 6 cases were "clonal dermatitis".Conclusion Clonal TCR γ-GR may be detected in patients with early CTCL,even when the histologic findings are not unequivocally diagnostic.

Key words: Lymphoma, T-cell, cutaneous, Genes, T-cell receptor gamma