Abstract: Objective To clone and study the antimicrobial-resistant gene in Neisseria gonorrhoeae.Methods The gene library,which contains the differential genes of antimicrobial resistant strains and standard reference strains of Neisseria gonorrhoeae,was constructed using a technique known as suppression subtractive hybridization(SSH).Then the antimicrobial resistance associated genes were cloned and analyzed.Results Subtractive gene library in antimicrobial-resistant Neisseria gonorrhoeae was successfully constructed,which contains 2500 positive clones.Sequence analysis was performed on5clones.The sequences of these five clones were unknown previously.Conclusions The subtractive DNA library is successively constructed which may provide an important clue for studying the mechanism of antimicrobial resistance in Neisseria gonorrhoeae.

Key words: Neisseria gonorrhoeae, Drug resistance,bacterial, Suppression subtractive hybridiza-tion, Sequence analysis,DNA, Gene library