Abstract: Objective To rapidly detect and identify pathogenic fungi of some deep fungal infections by PCR.Methods The suspensions of 22 pathogenic fungi(23strains)were amplified by PCR with fungal universal primers ITS86 and ITS4 which were labeled by FAM.The precise length of amplified fragments was determined by ABI PRISM^TM 377 Sequencer and Genescan analysis software,then compared with that of amplicons of corresponding fungal DNA which were previously extracted.Results (1)Amplification of 17 pathogenic fungi with ITS4,ITS86 resulted in a unique fragment length(except for A.nidulans and A.niger,C.albicans and C.stellatoidea,F.pedrosoi and E.dermatitidis).(2)No significant difference of the length of amplicons was found between the fungal suspension and control organisms,based on the results of Genescan analysis.(3)The whole process took only 6h to complete the detection.Conclusion The combination of fungal suspension PCR with ITS fungal universal primers and Genescan analysis might provide an accurate,specific,sensitive,and rapid approach to detect and identify 22 pathogenic fungi causing deep fungal infections,and hold promise to be applied for the diagnosis of deep fungal infection.

Key words: Mycoses, Sequence analysis,protein, Yeasts, Aspergillus, Phialophora, Sporothrix, Cladosporium, Mucor, Fusarium