银屑病患者骨髓CD34+细胞Notch受体及其靶基因表达的研究

Abstract

Abstract:

Objective To study the expressions of Notch receptors （Notch1 and 2） and target gene Hes-1 in bone marrow CD34+ cells from patients with psoriasis. Methods CD34+ cells were isolated from the bone marrow of 12 patients with psoriasis and 19 normal human controls. Western blot was conducted to detect the expressions of Notch1, Notch2 and Hes-1 proteins in CD34+ cells, with the house-keeping gene β-actin as an internal reference. Results In contrast with normal controls, a significant increment was observed in the expressions of Notch1 and Hes-1 proteins in psoriatic patients （0.9488 ± 0.3221 vs. 0.6693 ± 0.1465, 0.8579 ± 0.3729 vs. 0.5728 ± 0.1787, both P < 0.05）. No significant difference was observed in the expression of Notch2 between psoriatic patients and normal controls （0.7568 ± 0.3461 vs. 0.8312 ± 0.2887, P > 0.05）. Conclusion The over expression of Notch1 and Hes-1 may be associated with the low proliferation activity of psoriatic hematopoietic cells.

Key words: Hes-1

References

1. 牛旭平，李新华，张开明等. 银屑病患者骨髓造血细胞体外增殖活性研究. 中国皮肤病性病杂志，2007,21（04）：196-198. 2. Zhang Kaiming, Li Xinhua, Yin Guohua, et al. Functional characterization of CD4+CD25+ regulatory T cells differentiated in vitro from bone marrow-derived haematopoietic cells of psoriasis patients with a family history of the disorder. British Journal of Dermatology, 2008, 158:298–305. 3. 牛旭平，徐海涛，尹国华等.银屑病患者单一核细胞培养上清对骨髓造血干细胞和祖细胞集落形成的影响.中华皮肤科杂志,2006,39:143-145. 4. 尹国华，李新华，张开明等. 银屑病患者骨髓CD34+细胞体外定向分化T细胞的研究. 中华皮肤科杂志，2006，39：124-127. 5. Zhang K, Zhang R, Li X, et al. The mRNA expression and promoter methylation status of the p16 gene in colony-forming cells with high proliferative potential in patients with psoriasis. Clinical and Experimental dermatology, 2007, 32(2): 702-708. 6. K. Zhang, R. Hou, X. Niu, et al.Decreased colony formation of high proliferative potential colony-forming cells and granulocyte-macrophage colony-forming units and increased Hes-1 expression in bone marrow mononuclear cells from patients with psoriasis. British Journal of Dermatology, 已定稿（BJD9790）. 7. Artavanis-Tsakonas S, Matsuno K, Fortini ME: Notch signaling. Science. 1995, 68:225. 8. Egan SE, St Pierre B, Leow CC: Notch receptors, partners and regulators: From conserved domains to powerful functions. Curr Top Microbiol Immunol.1998, 228: 273. 9. Milner LA and Bigas A. Notch as a Mediator of Cell Fate Determination in Hematopoiesis: Evidence and Speculation. Blood, 1999, 93(8): 2431-2448. 10. Castella P, Sawai S, Nakao K, et al. HES-1 Repression of Differentiation and Proliferation in PC12 Cells: Role for the Helix 3-Helix 4 Domain in Transcription Repression. Molecular and Cellular Biology, 2000, 20(16): 6170-6183. 11. 侯瑞霞，尹国华，李新华等．银屑病患者骨髓CD34+细胞Notch受体的表达．中华微生物学和免疫学杂志，2009，29：212． 12. 侯瑞霞，尹国华，李俊琴等．银屑病患者骨髓CD34+细胞Hes-1基因的表达研究．临床皮肤科杂志，2009，38，619-621． 13. 李新华; 张开明; 尹国华等. 银屑病患者骨髓CD34+细胞定向分化的T细胞对角质形成细胞增殖的影响. 临床皮肤科杂志,2008,37(6):348-350. 14. Li Xinhua, Fan Xing, Zhang Kaiming, et al. In?uence of psoriatic peripheral blood CD4+ T and CD8+ T lymphocytes on C-myc, Bcl-xL and Ki67 gene expression in keratinocytes. Eur J Dermatol 2007; 17 (5): 1-5. 15. 张开明,李新华. 骨髓,银屑病的发病“中枢”? 中国皮肤性病学杂志, 2004,18：501-503. 16. Milner LA, Kopan R, Martin DI, et al. A human homologue of the Drosophila developmental gene, Notch, is expressed in CD34+ hematopoietic precursors. Blood 83:2057, 1994 17. Lam LT, Ronchini C, Norton J, et al. Suppression of Erythroid but Not Megakaryocytic Differentiation of Human K562 Erythroleukemic Cells by Notch-1. J. Biol. Chem., 2000, 275, (26): 19676-19684. 18. Neves H, Weerkamp F, Gomes AC. et al. Effects of Delta1 and Jagged1 on Early Human Hematopoiesis: Correlation with Expression of Notch Signaling-Related Genes in CD34+ Cells. Stem Cells, 2006, 24 (5): 1328-1337. 19. Bigas A, Martin DIK, Milner LA: Notch1 and Notch2 inhibit myeloid differentiation in response to different cytokines. Mol Cell Biol 1998，18:2324. 20. Atsushi K, Shigeru C, Etsuko NY, et al. HES-1 preserves purified hematopoietic stem cells ex vivo and accumulates side population cells in vivo. Blood, 2003, 101(5): 1777-1783.

Expressions of Notch receptors and target genes in bone marrow CD34+ cells from patients with psoriasis

PDF

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 0

Metrics

Comments

Recommended 10