应用基因芯片技术筛选皮肤光老化相关基因

Abstract

Abstract:

Objective To detect differentially expressed genes between human normal skin and photoaging skin, and to investigate the molecular and biological mechanisms of human skin photoaging at transcriptional level. Methods Full-thickness skin specimens were obtained during full-face rhytidectomy from sun-exposed （anterior ear skin） and sun-protected （retroauricular skin） sites of 6 patients with facial photoaging from 2007 to 2008. Genomic microarray analysis was conducted to identify differentially expressed genes between the two groups of specimens followed by gene-cluster analysis. Results The normalization of microarray data showed that the number of differentially expressed genes was 2163 between skin samples from sun-exposed and sun-protected sites in one patient, significantly higher than that in the other 5 patients （less than 200）; therefore, the data from the patient with 2163 differentially expressed genes were excluded from further analysis. Totally, 172 differentially expressed genes were identified with Beadarray chip, including 99 up-regulated genes and 73 down-regulated genes. Based on Genebank research, 118 functionally classified genes were found, which were associated with a series of biological processes, including cell adhesion, receptor regulation, signal transduction, metabolism, and so on. Conclusions There are a lot of differentially expressed genes between human photoaging skin and normal skin. Rhytidectomy may be associated with the differential expression of skin photoaging-related genes.

Key words: Matrix Metalloproteinases

References

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Screening of genes associated with skin photoaging by microarray analysis

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