雷公藤多苷对自身免疫性甲状腺炎大鼠模型外周血CXCR3、CCR4基因表达的影响

Abstract

Abstract:

Objective To analyze the impact of tripterygium glycosides （TG） on the expression of CXCR3 and CCR4 gene in peripheral blood mononuclear cells from experimental autoimmune thyroiditis （EAT） rats, and to explore the possible mechanism for regulation of Th1/Th2 balance by TG. Methods EAT was induced in 20 rats, which were randomly divided into two groups, i.e., control group and TG group, to receive intragastric physiological saline and TG suspension （5.5 mg/kg） daily, respectively. The rats were killed 4 weeks later, and total RNA was extracted from the peripheral blood mononuclear cells of rats and used for cRNA synthesis. The gene expressions of CXCR3 and CCR4 were detected with a Th1-Th2-Th3 microarray gene chip and realtime RT-PCR. Results Compared with the control group, there was a significant decrease in the mRNA expression of CXCR3 （0.52 ± 0.10 vs. 1.05 ± 0.17, P ＜ 0.01） and CXCR3/CCR4 ratio （0.39 ± 0.22 vs. 1.04 ± 0.12, P ＜ 0.01) in the TG group, together with an increase in the mRNA expression of CCR4 （1.56 ± 0.13 vs. 1.02 ± 0.09, P ＜ 0.01）. Conclusions TG could regulate the number of Th1 and Th2 cells as well as their cytokine expression ratio likely by reducing CXCR3 and enhancing CCR4 expression, so as to modulate the Th1/Th2 balance in EAT rats.

Key words: Tripterygium Wilfordii Polyglycoside, experimental autoimmune thyroiditis, Th1, Th2, gene chip, CXCR3, CCR4

References

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Impact of tripterygium glycosides on the expression of CXCR3 and CCR4 genes in peripheral blood from experimental autoimmune thyroiditis rats

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