Abstract:

Objective To genotype Trichosporon spp. with rDNA-ITS/IGS1-RFLP analysis followed by cluster analysis, and attempt to apply this method to rapid species identification of human pathogenic Trichosporon spp.. Methods Fourteen strains of Trichosporon, which belonged to 8 species, were collected. The rDNA-ITS/IGS1 regions were amplified by PCR and sequenced. Simultaneously, the amplicons were digested separately with restriction enzymes, including HaeⅢ, HhaⅠ, HaeⅢ and HhaⅠ, HinfⅠ, MspⅠ and TaqⅠ. Results The 8 species of Trichosporon could be classified into 4 subgroups with rDNA-ITS-RFLP, while interspecies identification of all the 14 strains from 8 species of Trichosporon could be realized with rDNA-IGS1-RFLP. Also, those genotypes of T. asahii which had relative long phylogenic distance could even be discriminated with rDNA-IGS1-RFLP. Conclusion The rDNA-ITS/IGS1-RFLP analysis is expected to be used in rapid interspecific identification of genus Trichosporon.

Key words: Trichosporon, Interspecific identification, ribosome DNA, PCR-RFLP