Abstract:

Objective To study the effects and the action mechanisms of six kinds of crude drugs (CORTEX DICTAMNI, FLOS LONICERAF, RADIX ANGELICAE SINENSIS, HERBA SCHIZONEPETAE, RADIX REHMANNIAE, RADIX ANGELICAE DAHURICAE) in murine model of allergic contact dermatitis. METHODS Mice were divided into eight groups according to different treatments: CORTEX DIC-TAMNI, FLOS LONICERAE, RADIX ANGELICAE SINENSIS, HERBA SCHIZONEPETAE, RADIX REH-MANNIAE, RADIX ANGELICAE DAHURICAE groups, dexamethasone (DEX) and saline groups (con-trol). Murine allergic contact dermatitis was induced by 2, 4-dinitrofluorobenzene. The eight ex-perimental groups were compared to the control group according to right ear thickness (S1), two ears thickness (S2), right ear weight (S3), the expression of interferon-γ (IFN-γ) as well as inter-leukin-10 (IL-10). RESULTS Compared with saline group, the right ear swelling， the number of infiltrated inflammatory cells in skin，and serum levels of IFN-γ were significantly reduced in CORTEX DICTAMNI , FLOS LONICERAE , RADIX ANGELICAE SINENSIS, HERBA SCHIZONEPETAE, and DEX groups .CONCLUSION CORTEX DICTAMNI , FLOS LONICERAE , RADIX ANGELICAE SINENSIS, HERBA SCHIZONEPETAE can significantly suppress murine allergic contact dermatitis and could be involved in the upregulation of IFN-γ expression. [KEYWORDS] Chinese materia medica; Dermatitis, allergic contact; IFN-γ