Abstract:

[Abstract]: Objective to determine the effect and possible mechanism of different concentration of malassezia furfur on the proliferation of keratinocytes. Methods MTT assay was used to determine the effect of different concentration of malassezia furfur(the ratio of malassezia furfur and HaCaT cells as 0:1;1:1;5:1;10:1;20:1;30:1;40:1;60:1;80:1;100:1) on the proliferation of HaCaT cells. Migration assay was also used to determine the antagonism effect of 10μM YC-1（pecific inhibitor of HIF-1α）, 2μM CBO-P11（pecific inhibitor of VEGF）, 5μg/ml MAB3571（neutralizing antibody of VEGFR-2）on the proliferation of HaCaT cells promoted by malassezia furfur(20:1). Westen blotting was used to determine the protein expression of HIF-1α, VEGF, VEGFR-2 . Results Low concentration of malassezia furfur promoted the proliferation of HaCaT cells, while high concentration of malassezia furfur promoted the apoptosis and necrosis of HaCaT cells. The promoted proliferation of HaCaT cells by malassezia furfur (20:1) was inhibited by YC-1（10μM）, CBO-P11（2μM）or MAB3571（5μg/ml）. Malassezia furfur (20:1) induced the protein expression of HIF-1α, VEGF, VEGFR-2, which was antagonized by YC-1（10μM）. Conclusions Low concentration of malassezia furfur can promote the proliferation of HaCaT cells through the activation of HIF-1α/VEGF/VEGFR-2 pathway.