Abstract:

Objective To evaluate the susceptibility of three strains of M. abscessus to antimicrobial agents. Methods Clinical Laboratory Standard Institute Broth microdilution method M24-A was applied to test the susceptibility of M. abscessus strains to amikacin, doxycycline, clarithromycin, sulfamethoxine, levofloxacin, clofazimine and dapsone. Results MIC ranges of three strains of M. abscessus were: amikacin 32μg/ml, doxycycline more than 32μg/ml, clarithromycin 1~8μg/ml, sulfamethoxine more than 128μg/ml, levofloxacin 16~32μg/ml, clofazimine 1~8μg/ml, dapsone more than 128μg/ml. Of the three strains, only one was sensitive to clarithromycin and clofazimine. Conclusion CLSI M24-A microdilution method is reliable, practical and easy to use in the clinical laboratory. The sensitivity of various strains of M. abscessus to the above mentioned antimicrobial agents is different.