Abstract:

Objective To observe the effect of endothelin-1 （ET-1） on the cell growth, adhesion, migration and expression of intercellular adhesion molecule 1 （ICAM-1） by A375 human malignant melanoma cells. Methods A375 cells were cultured in the presence of ET-1 of various concentrations （0.002, 0.02, 0.2, 2 μg/mL） for different periods. MTT method and flow cytometry were applied to detect the proliferation and ICAM-1 expression of these cells, respectively, after 24-, 48-, and 72-hour treatment. After 24-hour treatment, the cell adhesion and migration of A375 cells were assessed with cell adhesion assay and Transwell chambers, respectively. Results In the case of ET-1 from 0.002 to 0.2 μg/mL, it enhanced the proliferation, adhesion, migration of A375 cells and inhibited the expression of ICAM-1 by A375 cells in a dose dependent manner （P < 0.01 or < 0.05）; however, for ET-1 of 2 μg/mL, the situation was the opposite. Moreover, after 24-hour culture with ET-1 of 0.2 μg/mL, the metabolic activity, cell adhesion rate, and expression of ICAM-1 peaked at 0.327 ± 0.009, （163.31 ± 4.05）% and 4.667 ± 0.551, respectively. Conclusion ET-1 may enhance cellular metabolism and pigmentation by suppressing the expression of ICAM-1 and promoting the proliferation, adhesion and migration of melanoma cells.

Key words: A375 human melanoma cells;Endothelin-1;Intercellular adhesion molecule－1