Chinese Journal of Dermatology ›› 2008, Vol. 41 ›› Issue (12): 803-805.
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Abstract: Objective To observe the effect of several antihistamines, including epinastine, on the release of leukotriene B4 (LTB4), interleukin 4 (IL-4) and interleukin 5 (IL-5) from spleen lymphocytes of allergic mice challenged by ovalbumin (OVA). Methods OVA-sensitized BALB/c mice were used as animal model of allergy. Spleen lymphocytes isolated from these mice were treated with epinastine (0.1, 1.0, 10 μmol/L), ebastine (10 μmol/L), cetirizine (10 μmol/L), and dexamethasone (1.0 μmol/L), respectively, followed by incubation with OVA. Those cells without pretreatment with antihistamines served as the control. One hour after the challenge, enzyme-linked immunosorbent assay (ELISA) was applied to measure the level of LTB4, IL-4 and IL-5 in the cell culture supernates. Results Following the incubation with OVA, the levels of LTB4, IL-4 and IL-5 increased significantly in culture supernates of control spleen lymphocytes. Epinastine significantly inhibited the release of LTB4, IL-4 and IL-5 in a dose-dependent manner. At the concentration of 10 μmol/L, the inhibitory effect of epinastine was more potent than that of ebastine and cetirizine for IL-5 and LTB4 release (all P < 0.05); for IL-4, the effect of epinastine was similar to that of ebastine (P > 0.05) but more potent than that of cetirizine (P < 0.05). Conclusion Epinastine has a potent anti-inflammatory effect.
Key words: leukotriene B4, epinastine
CLC Number:
. Comparative study on the inhibitory effect of several antihistamines on the release of inflammatory mediators from lymphocytes[J].Chinese Journal of Dermatology, 2008,41(12):803-805.
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