Abstract: Objective This study aim to screen the aptamer with high affinity and specificity for N . gonorrhoeae and set a base for establishing relative easy and quick laboratory detection methods. Methods ssDNA pools are used in the selection process,ssDNA is heat-denatured at 95℃ for 5 min ,and then cooled immediately to 4℃ in binding buffer.Selection is performed by incubating ssDNA pools with bacterial at room temperature for 25-30 minute in binding buffer by gentle rotation.After 30 min the aptamers-bacterial antigen complex is centrifuging and washed four times with washing buffer.ssDNA retained on the bacterial is elute after heat by 99℃ ddH2O. Selected ssDNAs are amplified by PCR and used for the next round selection. After round 10th ,