Abstract: Objective To study the relationship between M. genitalium and nongonococcal, non- chlamydial mucopurulent cervicitis. Methods A total of 226 patients with nongonococcal, nonchlamydial mucopurulent cervicitis and 118 healthy female volunteers were recruited in this study. Cervical samples were collected and M.genitalium was detected by PCR amplification of 16s rRNA and Pa genes. Information about socio-demographic characteristics, medical history, and sexual behaviors was gathered by questionnaire from both populations. Results The prevalence of M. genitalium infection was 11.06% （25/226） in patients with mucpurulent cervicitis, 0.85% （1/118） in the healthy controls; the difference was significant between the two groups（χ2 = 11.58, P < 0.001）. Single variant analysis on the 226 patients showed that the prevalence of M. genitalium infection was 27.78%, 16.36%, 18.28% and 14.12% in patients with ectopic pregnancy history, cervical inflammation, pelvic organ tenderness, 10 or more polymorphonuclear leukocytes （PMNs） per oil immersion field in cervical discharge, respectively, significantly higher than that in patients without ectopic pregnancy history, cervical inflammation or pelvic organ tenderness, and those with less than 10 PMNs per oil immersion field in cervical discharge （9.62%, 6.03%, 6.02% and 1.79%, all P < 0.05）. M.genitalium infection was also related to multiple sex partners and the presence of mucopurulent secretion in cervix （P < 0.001）. Conclusion The prevalence of M.genitalium infection is higher in patients with nongonococcal, nonchlamydial mucopurulent cervicitis attending STD clinic than that in normal population.