Abstract: Objective To investigate the application of autologous fat globules transplantation in the repair of skin depression or defect. Methods Seventeen patients with skin depression or defect caused by various reasons and localized, at different body areas, were managed with autologous fat globules transplantation. A total of 2 - 130 mL autologous fat globules were injected every time with an excess of 30% over the original depression or defect volume. Patients were given 1 - 5 times of injection with an interval of 3 - 4 months. All patients were followed up for 6 - 12 months. Results The skin depression or defect in the faces, breasts and arms was treated successfully with the transplantation. Though about 30% of the fat globules could be absorbed after each transplantation, both patients and surgeons were satisfied with the effects after repeated injection. Conclusion Autotransplantation of fat globules is a safe, effective and convenient approach to repair skin depression or defect.