Chinese Journal of Dermatology ›› 2021, Vol. 54 ›› Issue (6): 469-474.doi: 10.35541/cjd.20201011

• Original Articles • Previous Articles     Next Articles

Effects of resveratrol on expression of inflammatory cytokines and related genes in benzo（a）pyrene-induced human sebocytes

Wei Ziyu1, Hou Xiaoxiao1, Cao Ke1, Li Xin1, Ye Feng1, Hu Tingting1, Mo Xiaohui1, Chen Guangjie2, Christos C. Zouboulis3, Ju Qiang1   

  1. 1Department of Dermatology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China; 2Department of Immunology and Microbiology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China; 3Departments of Dermatology, Venereology, Allergology and Immunology, Dessau Medical Center, Brandenburg Medical School Theodore Fontane, Dessau 06847, Germany
  • Received:2020-10-15 Revised:2020-11-18 Online:2021-06-15 Published:2021-05-31
  • Contact: Ju Qiang E-mail:qiangju401@sina.com
  • Supported by:
    National Natural Science Foundation of China （81874247, 81903234）; Scientific Research Project of Shanghai Science and Technology Commission （19ZR1431300）; Scientific Research Project of Shanghai Health and Family Planning Commission （20184Y0160）

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Abstract: 【Abstract】 Objective To investigate the effect of resveratrol on the expression of inflammatory cytokines and related genes in human SZ95 sebocytes induced by benzo（a）pyrene. Methods Human SZ95 sebocytes were cultured in vitro, and divided into 4 groups: control group treated with 1‰ dimethyl sulfoxide for 27 hours, resveratrol group treated with 1 × 10-5 mol/L resveratrol for 24 hours, benzo（a）pyrene group treated with 1 × 10-5 mol/L benzo（a）pyrene for 3 hours, resveratrol + benzo（a）pyrene group treated with 1 × 10-5 mol/L resveratrol for 24 hours followed by 1 × 10-5 mol/L benzo（a）pyrene for 3 hours. Real-time fluorescence-based quantitative PCR was performed to determine the mRNA expression of interleukin（IL）-1α, IL-6, aryl hydrocarbon receptor （AhR）, cytochrome P4501A1（CYP1A1） and cytochrome P4501B1（CYP1B1） in SZ95 sebocytes in the above groups; Western blot analysis was conducted to determine the phosphorylation level of p38 mitogen-activated protein kinase （p38 MAPK, expressed as the ratio of phosphorylated to total p38 MAPK） and AhR protein expression; enzyme-linked immunosorbent assay （ELISA） was conducted to detect levels of IL-1α and IL-6 in the cell culture supernatant in each group. One-way analysis of variance was used for comparison of means among multiple groups, and least significant difference-t test was used for multiple comparisons. Results The mRNA and protein expression of IL-1α in SZ95 sebocytes significantly differed among the control group, resveratrol group, benzo（a）pyrene group and resveratrol + benzo（a）pyrene group （mRNA: 2.045 ± 0.272, 2.058 ± 0.154, 3.124 ± 0.094, 2.185 ± 0.337, protein: 9.132 ± 1.181, 9.429 ± 0.771, 20.361 ± 0.907, 9.917 ± 0.897, F = 14.662, 101.705, P < 0.01, < 0.001, respectively）, and were significantly lower in the resveratrol + benzo（a）pyrene group than in the benzo（a）pyrene group （both P < 0.01）. In addition, the phosphorylation level of p38 was significantly higher in the benzo（a）pyrene group than in the control group, resveratrol group and resveratrol + benzo（a）pyrene group （F = 303.129, P < 0.000 1）. The mRNA expression of AhR, CYP1A1 and CYP1B1 was significantly lower in the resveratrol + benzo（a）pyrene group than in the benzo（a）pyrene group （t = 10.64, 33.599, 18.327, respectively, all P < 0.001）. The benzo（a）pyrene group showed significantly decreased protein expression of AhR compared with the resveratrol + benzo（a）pyrene group （P < 0.001）. Conclusion Resveratrol can inhibit the environmental pollutant benzo（a）pyrene-induced expression of inflammatory factor IL-1α in SZ95 sebocytes, which is likely mediated by the AhR and p38MAPK pathways.

Key words: Sebaceous glands, Acne, Benzo（a）pyrene, p38 Mitogen-activated protein kinases, Interleukin-1alpha, Resveratrol, Human SZ95 sebocytes, Aryl hydrocarbon receptor