Abstract: He Chengyan, Sun Yi, Gao Lujuan, Li Ming, Zeng Tongxiang Department of Dermatology, Jingzhou Central Hospital, The Second Clinical Medical College, Yangtze University, Jingzhou 434100, Hubei, China （He CY, Sun Y, Zeng TX）; Department of Dermatology, Zhongshan Hospital, Fudan University, Shanghai 200032, China （Gao LJ, Li M） Corresponding authors: Gao Lujuan, Email: gao_lujuan@163.com; Sun Yi, Email: jzzxyysy@163.com 【Abstract】 Objective To evaluate in vitro antifungal activity of tacrolimus combined with itraconazole or terbinafine against Exophiala dermatitidis （E. dermatitidis）. Methods The minimum inhibitory concentrations （MICs） of itraconazole and terbinafine against 12 strains of E. dermatitidis were determined using the Clinical and Laboratory Standards Institute （CLSI） broth microdilution susceptibility method （M38?A2 Document）. A broth microdilution checkerboard method was used to evaluate the in vitro antifungal activity of tacrolimus combined with itraconazole or terbinafine against E. dermatitidis. Results The MIC ranges of terbinafine and itraconazole against E. dermatitidis were 0.060 - 0.125 mg/L and 0.5 - 1 mg/L, respectively. The combination of tacrolimus with terbinafine showed synergistic inhibitory effects against 5 strains of E. dermatitidis, while the combination of tacrolimus with itraconazole revealed synergistic effects against 10 strains of E. dermatitidis. No antagonism was observed in either of the two combinations. Conclusion In vitro combination of tacrolimus with itraconazole or terbinafine can enhance the antifungal activity of itraconazole or terbinafine against E. dermatitidis.