Abstract: Han Ying, Li Tienan, Liu Wenli, Jin Chunlin, Cheng Xin, Liu Yi, He Sha, Wu Shandong Department of Dermatology, Shenyang No.7 People′s Hospital, Shenyang 110003, China （Han Y, Li TN, Liu WL, Jin CL, Cheng X）; Hangzhou Zheda Dixun Biological Gene Engineering Co., Ltd, Hangzhou 310052, China （Liu Y, He S, Wu SD） Corresponding author: Li Tienan, Email: qiyuanmys@163.com 【Abstract】 Objective To investigate relationships between serum levels of anti?tyrosinase IgG antibody （TYR IgG） as well as anti?tyrosinase?related protein?1 IgG antibody （TRP?1 IgG） and vitiligo. Methods Enzyme linked immunosorbent assay （ELISA） was performed to detect serum levels of TYR IgG and TRP?1 IgG in 260 patients with vitiligo and 50 health controls. The threshold for defining a positive test result was set at 3 standard deviations above the mean serum level of TYR IgG or TRP?1 IgG in the healthy controls. Results The positive rate of TYR IgG and/or TRP?1 IgG in the vitiligo group was 57.31% （149/260）. The positive rates of TYR IgG and TRP?1 IgG were both significantly higher in the vitiligo group than in the control group （TYR IgG: 37.3%［97/260］ vs. 0, χ2 = 25.441, P < 0.01; TRP?1 IgG: 33.5%［87/260］ vs. 0, χ2 = 21.630, P < 0.01）. The positive rate of TYR IgG was not associated with that of TRP?1 IgG in the vitiligo group （r = -0.032, P > 0.05）. Among patients with vitiligo, the positive rate of TRP?1 IgG was significantly higher in females than in males （χ2 = 5.811, P < 0.05）, as well as in patients aged ≤ 20 years than in those aged > 20 years （χ2 = 6.498, P < 0.05）, while the positive rate of TYR IgG didn′t differ between females and males, or between patients aged ≤ 20 years and those aged > 20 years （both P > 0.05）. Conclusion Detection of TYR IgG and TRP?1 IgG may provide some evidence for the diagnosis and treatment of vitiligo.