Abstract: Tang Yaping, Sheng Wenting, Zhou Xin, Li Xiaodong, Tian Xin, Zhong Jinbao, Li Zhenjie, Lin Chunsheng, Liu Yumei Department of Dermatology, Guangzhou Institute of Dermatology, Guangzhou 510095, China （Tang YP, Zhou X, Li XD, Tian X, Zhong JB, Li ZJ, Lin CS, Liu YM）; Department of Dermatology, Guangzhou Red Cross Hospital, Guangzhou 510220, China （Sheng WT） Corresponding author: Tang Yaping, Email: tangya1130@126.com 【Abstract】 Objective To compare of Toll-like receptors 7 and 9 （TLR7, TLR9） as well as their regulatory molecules myeloid differentiation factor 88 （MyD88） and nuclear factor-κB （NF-κB） in peripheral blood mononuclear cells （PBMCs） between patients with vitiligo and healthy individuals, and to explore their significance. Methods Flow cytometry was performed to measure of TLR7 and TLR9 in PBMCs among 36 patients with vitiligo and 22 healthy controls, and real-time fluorescence-based quantitative PCR （RT-PCR） was conducted to determine mRNA of MyD88 and NF-κB in the above blood samples. Results Compared with healthy controls, patients with vitiligo showed higher of TLR7 and mRNA of MyD88 and NF-κB, but lower of TLR9. However, significant differences were only observed in the mRNA of NF-κB （t = 2.814, P = 0.008）, but not in the of TLR7 and TLR9 or the mRNA of MyD88 between patients and controls （t = 1.477, 1.761, 0.058, all P > 0.05）. Conclusion NF-κB, as a key signaling molecule of TLR7 and TLR9 regulation pathways, increases obviously in patients with vitiligo, suggesting that NF-κB may be involved in the pathogenesis of vitiligo.