Abstract:

Ge Xinhong, Tang Zhenzhen, Jiao Yaning, Wu Hao, Yu Nan, Dong Lingdi, Li Le, Yang Biao, Pu Xiaoxia Department of Dermatology, General Hospital of Ningxia Medical University, Yinchuan 750004, China （Ge XH, Jiao YN, Yu N, Dong LD, Li L）; Department of Dermatology, Lanling People′s Hospital, Linyi 277700, Shandong, China （Tang ZZ）; Ningxia Medical University, Yinchuan 750004, China （Wu H, Yang B, Pu XX） Corresponding author: Ge Xinhong, Email: gexinhong.0101@163.com 【Abstract】 Objective To investigate s of phosphorylated c-Jun N-terminal kinase （p-JNK） and P38 mitogen-activated protein kinase （p-P38MAPK） in psoriasis vulgaris lesions. Methods Tissue specimens were obtained from lesions of 30 patients with psoriasis vulgaris and normal skin of 30 healthy human controls. An immunohistochemical study and Western-blot analysis were performed to measure protein s of p-JNK and p-P38MAPK in these skin specimens. Results As the immunohistochemical study showed, the s of p-JNK and p-P38MAPK （expressed as the average optical density ［AOD］ value for targeted proteins） were significantly higher in psoriasis vulgaris lesions than in normal skin tissues （p-JNK: 0.663 ± 0.016 vs. 0.333 ± 0.009, t = 44.869, P < 0.001; p-P38MAPK: 0.436 ± 0.011 vs. 0.306 ± 0.010, t = 21.913, P < 0.001）. Western-blot analysis also showed increased protein s of p-JNK and p-P38MAPK in psoriasis vulgaris lesions compared with normal skin tissues （t = 20.477, 165.084, respectively, both P < 0.05）. Conclusion The activation of JNK and P38MAPK may be involved in the overproliferation of epidermal cells in psoriasis vulgaris lesions.