左旋多巴对马尔尼菲青霉黑素合成以及药敏的影响

Abstract

Abstract:

Peng Cheng, Sun Xian, Liu Donghua. Department of Dermatology and Venereology, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China Corresponding author: Liu Donghua, Email: ldhgxmu@163.com 【Abstract】 Objective To investigate the effect of levodopa on melanogenesis in Penicillium marneffei （PM）, and to determine if melanization affects the antifungal drug susceptibility of PM. Methods A clinical isolate of PM, GXMU121011, was inoculated onto Sabouraud′s dextrose agar （SDA） containing different concentrations （0.1 - 10 mmol/L） of levodopa at an inoculum density of 1.0 × 106 cfu/ml, or onto SDA containing 1 mmol/L levodopa at three inoculum densities （1.0 × 105, 1.0 × 106, 1.0 × 107 cfu/ml）, and cultured at 37 ℃ for 7 days. Subsequently, melanization of PM colonies was observed. The paper-disk method was used for antifungal susceptibility testing, and the diameter of inhibition zones of itraconazole, fluconazole and amphotericin B against 8 clinical strains of PM was determined on SDA with or without levodopa. Results The melanization of PM colonies increased when the concentration of levodopa increased from 0.1 to 1.0 mmol/L, peaked when that reached 1.0 and 3.0 mmol/L, but mildly decreased when that continuously increased beyond 3.0 mmol/L, and a slight shrinkage was observed in PM colonies when that was 10.0 mmol/L. The color of colonies deepened along with the increase in inoculum density of PM. The average diameters of inhibition zones of itraconazole, fluconazole and amphotericin B against PM were all significantly lower on SDA with levodopa than on SDA without levodopa （all P < 0.05）. Conclusions Levodopa concentration and inoculum density both affect melanogenesis in PM. Melanization may decrease the susceptibility of PM in yeast phase to itraconazole, fluconazole and amphotericin B in vitro.

CLC Number:

R756.6

References

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Effect of levodopa on melanogenesis in and antifungal drug susceptibility of Penicillium marneffei

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