Abstract: Objective To investigate the effects of intense pulsed light(IPL)on the cell viability of and secretion of MMP-1 and MMP-2 by cultured human fibroblasts.Methods Fibroblasts were isolated from healthy infant foreskins,and irradiated with 18,29,35 J/cm² of IPL respectively.The morphological changes of cells were observed by light microscopy.MTT assay was used to detect the proliferation and viability of the fibroblasts.The secretion levels of MMP-1 and MMP-2 were determined by ELISA.Results IPL irradiation showed no noticeable effects on the morphology of fibroblasts.Proliferation of fibroblasts was increased at 24 h and 48 h after IPL irradiation of 18,29,35 J/cm²;however,no correlation with the irradiation dose was observed.IPL irradiation showed no effect on MMP-1 and MMP-2 secretion by the fibroblasts. Conclusion IPL irradiation could induce the proliferation of cultured human fibroblasts.

Key words: Intense pulse light, Fibroblasts, Matrix metalloproteases